Recombinant Anti-p27 KIP 1 antibody (Rabbit mAb)
   

Recombinant Anti-p27 KIP 1 antibody (Rabbit mAb)

Antibodies Oncology
AO-03-GB15517-50
CDKN1B,CDKN4,KIP1,MEN1B,MEN4,P27KIP1,cyclin-dependent kinase inhibitor 1B,cyclin dependent kinase inhibitor 1B,p27Kip1,Cdki1b,p27,Cyclin-dependent kinase inhibitor p27,P27-like cyclin-dependent kinase inhibitor
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Product Information

Protein full name

Cyclin-dependent kinase inhibitor 1B

Synonyms

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, cyclin-dependent kinase inhibitor 1B, cyclin dependent kinase inhibitor 1B,p27Kip1, Cdki1b, p27, Cyclin-dependent kinase inhibitor p27, P27-like cyclin-dependent kinase inhibitor

Immunogen

Recombinant protein corresponding to Human p27 KIP 1

Uniprot ID

P46527

Isotype

IgG, κ

Clone Number

SB289

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm, Endosome

Predicted MW. / Observed MW.

22 kDa / 27 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, brain, HeLa

IHC/IF

Human, Rat

1: 500-1: 1000

colon cancer, brain, tonsil

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Images

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WB analysis of p27 KIP 1(GB15517).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of p27 KIP 1(GB15517).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of p27 KIP 1(GB15517)red.
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

For Research Use Only!


: AO-03-GB15517-50
: 10 Items
Hurry! only 10 items left in stock.

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