Recombinant Anti-CDKN2A/p16INK4a antibody (Rabbit mAb)
   

Recombinant Anti-CDKN2A/p16INK4a antibody (Rabbit mAb)

Antibodies Oncology
AO-03-GB151143-100
Cyclin-dependent kinase 4 inhibitor A,CDK4I,CDKN2A,MTS1,CDKN2,Multiple tumor suppressor 1,MTS-1,p16-INK4a,p16INK4A,p16-INK4,p16-INK4a,Cdkn2a,P16ink4a
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Product Information


Protein full name

Cyclin-dependent kinase inhibitor 2A

Synonyms

Cyclin-dependent kinase 4 inhibitor A, CDK4I, CDKN2A, MTS1, CDKN2 , Multiple tumor suppressor 1, MTS-1, p16-INK4a, p16INK4A, p16-INK4, p16-INK4a, Cdkn2a, P16ink4a

Uniprot ID

P42771

Immunogen

KLH conjugated Synthetic peptide corresponding to Human CDKN2A/p16INK4a

Isotype

IgG

Clone Number

SB383

Predicted MW./Observed MW.

16 kDa / 16 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 1000-1: 2000 SiHa, 293T, HeLa
IHC/IF Human 1: 1000-1: 3000 cervical carcinoma



Background

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.



Images

01t3bqbhNKtOVzh.jpg

 WB analysis of CDKN2A/p16INK4a(GB151143).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02eieqKMcK22I1K.jpg

 WB analysis of CDKN2A/p16INK4a(GB151143).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03UGWUi0LxBwUbf.jpg

 IHC analysis of CDKN2A/p16INK4a(GB151143).
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

04nhM7xMchtIOI5.jpg

 IHC analysis of CDKN2A/p16INK4a(GB151143).
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

05XVhFatACPQpNz.jpg

 IHC analysis of CDKN2A/p16INK4a(GB151143).
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

06Hxt5obb1W6dU2.jpg

 IHC analysis of CDKN2A/p16INK4a(GB151143).
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

077OV2UD6S4kO5E.jpg

 IF analysis of CDKN2A/p16INK4a(GB151143)red.
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08jdFbtwjmmrBvB.jpg

 IF analysis of CDKN2A/p16INK4a(GB151143)red.
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

09GKjgTWQ0Lz7hQ.jpg

 IF analysis of CDKN2A/p16INK4a(GB151143)red.
Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB151143-100
: 10 Items
Hurry! only 10 items left in stock.

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