Recombinant Anti-TSG101 antibody (Rabbit mAb)

AO-03-GB150052-50
TSG10,TSG101,tumor susceptibility gene 101,VPS23,tumor susceptibility 101,Cell and organelle markers,ESCRT I complex subunit TSG101,Exosome marker
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Product Information


Protein full name

Tumor susceptibility gene 101 protein

Synonyms

TSG10, TSG101, tumor susceptibility gene 101, VPS23, tumor susceptibility 101, Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker

Uniprot ID

Q99816

Immunogen

Recombinant protein corresponding to Human TSG101

Isotype

IgG1, κ

Clone Number

SB333

Predicted MW./Observed MW.

44 kDa / 46 kDa

Purity

Affinity purification




Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 2000 K562, 293T, heart




Background

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).




Images

01QpZky6cJg8LsT.jpg

WB analysis of TSG101(GB150052).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02M4lQeX76uXPo3.jpg

WB analysis of TSG101(GB150052).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.




Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).


: AO-03-GB150052-50
: 10 Items
Hurry! only 10 items left in stock.

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