Recombinant Anti-CDK2 antibody (Rabbit mAb)

AO-03-GB150046-100
Cell division protein kinase 2,p33 protein kinase,CDK2,CDKN2,CDC28,CDC2A,Cdc2 related protein kinase,p33(CDK2),MPF
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Product Information


Protein full name

Cyclin-dependent kinase 2

Synonyms

Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2, CDC28, CDC2A,Cdc2 related protein kinase, p33(CDK2), MPF

Uniprot ID

P97377

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cdk2

Isotype

IgG, κ

Clone Number

SB348

Predicted MW./Observed MW.

34 kDa / 34 kDa

Purity

Affinity purification




Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 2000 Hela, HepG2, testis




Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B).




Images

01I0DgEUjhRranC.jpg

WB analysis of CDK2(GB150046).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02T6Jxz3soSGYlp.JPG

WB analysis of CDK2(GB150046).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.




Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).


: AO-03-GB150046-100
: 10 Items
Hurry! only 10 items left in stock.

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