Recombinant Anti-CDK4 antibody (Rabbit mAb)
   

Recombinant Anti-CDK4 antibody (Rabbit mAb)

Antibodies Oncology
AO-03-GB151602-50
Cell division protein kinase 4,PSK-J3,CDK4,Crk3,Cyclin dependent kinase 4,MGC14458,p34 cdk4,PSK-J3,CMM3,cyclin-dependent
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Product Information


Protein full name

Cyclin-dependent kinase 4

Synonyms

Cell division protein kinase 4, PSK-J3, CDK4, Crk3, Cyclin dependent kinase 4, MGC14458, p34 cdk4, PSK-J3, CMM3, cyclin-dependent

Uniprot ID

P11802

Immunogen

Recombinant protein corresponding to Human CDK4

Isotype

IgG, λ

Clone Number

SB287

Predicted MW./Observed MW.

34 kDa / 34 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus, Membrane



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 2000 NIT3T3, PC-12, spleen
IHC/IF Human, Mouse, Rat 1: 1000-1: 2000 colon cancer, colon, spleen



Background

CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression.



Images

01IBC3BqG3fZ9F8.jpg

 WB analysis of CDK4(GB151602).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02eEcXsL8VzbUQr.jpg

 IHC analysis of CDK4(GB151602).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

03ynpY8pFAF5NaP.jpg

 IHC analysis of CDK4(GB151602).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

04X9OWsy6NDUbdN.jpg

 IHC analysis of CDK4(GB151602).
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

05h1sRZZhfessrq.jpg

 IHC analysis of CDK4(GB151602).
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

061HlHjZur6SVtJ.jpg

 IHC analysis of CDK4(GB151602).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB151602-50
: 10 Items
Hurry! only 10 items left in stock.

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