Anti-PDCD4 Rabbit pAb
   

Anti-PDCD4 Rabbit pAb

Antibodies Oncology
AO-03-GB11991-50

Dug,MGC33046,Nuclear antigen H731,Death up-regulated gene protein,11991,MGC33047,Protein 197/15a,Neoplastic transformation inhibitor,Protein MA-3,Tis,Ma3,RP11 348N5.4,Topoisomerase-inhibitor suppressed protein,Nuclear antigen H731-like,PDCD4,H731,GB11991,PDCD 4,PDCD-4,Programmed cell death protein 4

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Product Information

Protein full name

Programmed cell death protein 4

Synonyms

Topoisomerase-inhibitor suppressed protein, Death up-regulated gene protein, MGC33046, MGC33047, Protein MA-3, Ma3, Tis, Dug, H731, Neoplastic transformation inhibitor, Nuclear antigen H731, Protein 197/15a, RP11 348N5.4, Nuclear antigen H731-like

Uniprot ID

Q61823

Immunogen

Recombinant protein corresponding to Mouse PDCD4

Isotype

IgG

Predicted MW./Observed MW.

52 kDa / 64 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Applications

Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 spleen, thymus, colon
IHC/IF Human, Rat 1: 400-1: 1600 breast cancer, colon cancer, spleen

Background

The Pdcd4 gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells.Two transcripts encoding different isoforms have been identified. Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.

Images

01hOsbPVvtBNtfu.jpg

 WB analysis of PDCD4(GB11991).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02z7GGN1bprpRyH.jpg

 IHC analysis of PDCD4(GB11991).
Sample: Human breast cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

036J4llHyvTQA7D.jpg

 IHC analysis of PDCD4(GB11991).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04HgYgwPSYX5yEG.jpg

 IHC analysis of PDCD4(GB11991).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

051h1SO4QOU0FZd.jpg

 IHC analysis of PDCD4(GB11991).
Sample: Rat thymus, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB11991-50
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