Anti-Bub1 Rabbit pAb

Anti-Bub1 Rabbit pAb

Antibodies Oncology
AO-03-GB11979-50
Bub 1,BUB1,hBUB1 mitotic checkpoint serine/threonine kinase,Budding uninhibited by benzimidazoles 1 homolog,Bub1,BUB1L,11979,BUB1A,Bub-1,GB11979,Mitotic checkpoint serine/threonine-protein kinase BUB1
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Product Information


Protein full name

Mitotic checkpoint serine/threonine-protein kinase BUB1

Synonyms

BUB1, BUB1A, BUB1L, hBUB1 mitotic checkpoint serine/threonine kinase, Budding uninhibited by benzimidazoles 1 homolog, Bub1

Uniprot ID

O08901

Immunogen

Recombinant protein corresponding to Mouse Bub1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 1000-1: 4000 spleen, lung, testis



Background

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer.



Images

01NtOZLMZULU4jP.jpg

 IHC analysis of Bub1(GB11979).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02qF0pNJIUQtSrf.jpg

 IHC analysis of Bub1(GB11979).
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03VjFWmXPfwnnkQ.jpg

 IHC analysis of Bub1(GB11979).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11979-50
: 10 Items
Hurry! only 10 items left in stock.

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