Anti-p19 INK4d Rabbit pAb

Anti-p19 INK4d Rabbit pAb

Antibodies Oncology
AO-03-GB11419-50
p19-INK4d,CDKN2D,pI,INK 4,11419,INK-4d,p-19,p19 INK4d,p 19,Anti-P19 INK4d Rabbit pAb,Nur77 associating protein,cyclin dependent kinase inhibitor 2D,p19,INK4D,p19INK4d,INK 4d,GB11419,INK-4,GB11419100,CDK inhibitor p19INK4d,Cyclin-dependent kinase 4 inhibitor D
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Product Information


Protein full name

Cyclin-dependent kinase 4 inhibitor D

Synonyms

CDKN2D, INK4D, p19, p19 INK4d, cyclin dependent kinase inhibitor 2D, CDK inhibitor p19INK4d, Nur77 associating protein

Uniprot ID

Q60773

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse p19 INK4d

Isotype

IgG

Predicted MW./Observed MW.

19 kDa / 19 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 250-1: 1500 HeLa, HepG2, A431
ICC/IF Human 1: 100-1: 300 HeLa



Background

Cyclin-dependent kinase 4 inhibitor D is an enzyme that in humans is encoded by the CDKN2D gene.The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase.



Images

01AZI5060QSTu6E.jpg

 WB analysis of p19 INK4d(GB11419).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02bDN3FFdKOlylT.jpg

 ICC analysis of p19 INK4d(GB11419)red.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11419-50
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