Recombinant Anti-PLK1 antibody (Rabbit mAb)

Protein full name

Serine/threonine-protein kinase PLK1

Synonyms

PLK, STPK13, PLK1, Polo-like kinase 1 (PLK-1), Serine/threonine-protein kinase 13 (STPK13), 2.7.11.21, Serine/threonine-protein kinase PLK1

Immunogen

Recombinant protein corresponding to Human PLK1

Uniprot ID

P53350

Isotype

IgG

Clone Number

SB636

Purity

Affinity purification

Predicted MW./Observed MW.

68 kDa / 68 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1500

293, C6, RAW264.7

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis.

Images

WB analysis of PLK1(AO-03-GB150186).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 5000, RT, 1h.