Anti-PCNA Mouse mAb
   

Anti-PCNA Mouse mAb

Antibodies Oncology
AO-03-GB12010-50
12010,AO-03-GB12010,AO-03-GB12010100,ATLD2,PCNA,proliferating cell nuclear antigen,Proliferating cell nuclear antigen
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Product Information


Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antigen

Uniprot ID

P17918

Immunogen

Recombinant protein corresponding to Mouse PCNA

Isotype

IgG2a, κ

Clone Number

4C9A9

Predicted MW./Observed MW.

29 kDa / 36 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 testis, placenta
IHC/IF Human, Mouse, Rat 1: 1000-1: 4000 colon, rectum, spleen
ICC/IF Human, Mouse, Rat 1: 100-1: 500 HeLa



Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.



Images

01JaFr3gHFIrT8s.jpg

 WB analysis of PCNA(AO-03-GB12010).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 5000, RT, 1h.

025VPo0OEFtdYJK.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Human gastric cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

03pFACFvYiFXWy0.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Human rectum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

0455F5aKeNESBvg.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Mouse colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

05rkdpqMphM7mqn.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

06lYGmNSH22tr8W.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

07lCI4HnZbAlMC4.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

08i9jUYGqCA6VQS.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

09OUmWLWVx5j2bO.jpg

 IF analysis of PCNA(AO-03-GB12010)red.
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

10NajbOEeAFtLkN.jpg

 IF analysis of PCNA(AO-03-GB12010)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

11Yqp8BlZHWSlGS.jpg

 IF analysis of PCNA(AO-03-GB12010)red.
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

12kRtvnFgPfnNF8.jpg

 IF analysis of PCNA(AO-03-GB12010)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

13vq4FM7XZ3Drk5.jpg

 ICC analysis of PCNA(AO-03-GB12010)red.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

147AcAlensQRU3y.jpg

 ICC analysis of PCNA(AO-03-GB12010)red.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB12010-50
: 10 Items
Hurry! only 10 items left in stock.

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