Anti-Ki67 Rabbit pAb
   

Anti-Ki67 Rabbit pAb

Antibodies Oncology
AO-03-GB111499-50
KIA,MKI67,111499,PPP1R105,Ki67,AO-03-GB111499100,Ki-67,marker of proliferation Ki-67,MIB-1,Ki 67,AO-03-GB111499,Proliferation marker protein Ki-67,AO-03-GB13030-2,Ki67染色,Anti -Ki67 Rabbit pAb,G111499
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Product Information

Protein full name

Proliferation marker protein Ki-67

Synonyms

MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Ki67

Uniprot ID

P46013

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human, Mouse, Rat, pig

1: 500-1: 1000

colon cancer, esophagus, esophagus cancer

ICC/IF

Human, Mouse, Rat

1: 200-1: 400

HeLa

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility .

Images

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IHC analysis of Ki67(AO-03-GB111499).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 200, RT, 1h.

IHC analysis of Ki67(AO-03-GB111499).
Sample: Mouse ovary, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 200, RT, 1h.

IF analysis ofKi67(AO-03-GB111499)red.
Sample: Human intestinal cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.

For Research Use Only!                                               Ver. No.: V2.1-202560114


: AO-03-GB111499-50
: 10 Items
Hurry! only 10 items left in stock.

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