Anti-Ki67 Rabbit pAb
   

Anti-Ki67 Rabbit pAb

Antibodies Oncology
AO-03-GB111141-50
KIA,MKI67,AO-03-GB111141,PPP1R105,Ki67,111141,Ki-67,marker of proliferation Ki-67,AO-03-GB111141100,MIB-1,Ki 67,Proliferation marker protein Ki-67,AO-03-GB13030-2,Ki67染色,Anti -Ki67 Rabbit pAb,G111499
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Product Information


Protein full name

Proliferation marker protein Ki-67

Synonyms

MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105

Uniprot ID

E9PVX6

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Ki67

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 500-1: 1200 colon, placenta, subcutaneous transplant tumor（H22）



Background

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility .



Images

01tF6AEL7soYjbl.jpg

 IHC analysis of Ki67(AO-03-GB111141).
Sample: Mouse placenta, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 200, RT, 1h.

02K2LFt54HPthKu.jpg

 IHC analysis of Ki67(AO-03-GB111141).
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 750, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 200, RT, 1h.

03yRYKV26lG8bnD.jpg

 IF analysis of Ki67(AO-03-GB111141)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.

04L2QGh9fdIyLYw.jpg

 IF analysis of Ki67(AO-03-GB111141)red.
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111141-50
: 10 Items
Hurry! only 10 items left in stock.

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