Anti-Ki67 Mouse mAb

Anti-Ki67 Mouse mAb

Antibodies Oncology
AO-03-GB121142-100
KIA,MKI67,PPP1R105,Ki67,AO-03-GB121142100,Ki-67,marker of proliferation Ki-67,AO-03-GB121142,121142,MIB-1,Ki 67,Proliferation marker protein Ki-67,AO-03-GB13030-2,AO-03-GB11030,Ki67染色
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Product Information


Protein full name

Proliferation marker protein Ki-67

Synonyms

MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105

Uniprot ID

P46013

Immunogen

Human recombinant Ki67 protein expressed in HEK293 cells

Isotype

IgG1, κ

Clone Number

1C3B12

Predicted MW./Observed MW.

359 kDa / 359 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 NIH3T3
IHC/IF Human 1: 500-1: 1500 colon, tonsil



Background

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility .



Images

01hL6eIyhxIHTac.jpg

 WB analysis of Ki67(AO-03-GB121142).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 5000, RT, 1h.

02l6vebmquitvg5.jpg

 IHC analysis of Ki67(AO-03-GB121142).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

03z70JKTeKL5nVa.jpg

 IHC analysis of Ki67(AO-03-GB121142).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB121142-100
: 10 Items
Hurry! only 10 items left in stock.

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