Anti-Ki67 Mouse mAb
   

Anti-Ki67 Mouse mAb

Antibodies Oncology
AO-03-GB121141-100
KIA,MKI67,PPP1R105,Ki67,AO-03-GB121141,AO-03-GB121141100,Ki-67,marker of proliferation Ki-67,MIB-1,Ki 67,121141,Proliferation marker protein Ki-67,AO-03-GB13030-2,Ki67染色
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Product Information


Protein full name

Proliferation marker protein Ki-67

Synonyms

MKI67, marker of proliferation Ki-67, KIA,MIB-1, PPP1R105

Uniprot ID

P46013

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Ki67

Isotype

IgG1, κ

Clone Number

6D12B2

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human, Mouse, Rat 1: 300-1: 600 esophageal cancer, ovarian cancer, spleen
ICC/IF Human, Mouse, Rat 1: 100-1: 500 HeLa, NIH/3T3



Background

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility .



Images

01k0ilTqFWSCZay.jpg

 IHC analysis of Ki67(AO-03-GB121141).
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

02H5jL6WDSCyu1O.jpg

 IHC analysis of Ki67(AO-03-GB121141).
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

03c3wTkhQO6ZQSq.jpg

 IHC analysis of Ki67(AO-03-GB121141).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

04SmNB7BDDWjEag.jpg

 IHC analysis of Ki67(AO-03-GB121141).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

05Mv98T2C2ftomO.jpg

 IHC analysis of Ki67(AO-03-GB121141).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

06Itgg4zu5PXISs.jpg

 IHC analysis of Ki67(AO-03-GB121141).
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

07ze1Q76bHOYo3x.jpg

 IHC analysis of Ki67(AO-03-GB121141).
Sample: Human colorectal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

08RJBBXrsb8xCWC.jpg

 IHC analysis of Ki67(AO-03-GB121141).
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

09kxckbUMg5CsrM.jpg

 IF analysis of Ki67(AO-03-GB121141)red.
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

10gnw6WB8MhFKBX.jpg

 IF analysis of Ki67(AO-03-GB121141)red.
Sample: Rat small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

11dekHueh0FdwJC.jpg

 IF analysis of Ki67(AO-03-GB121141)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

12xeF8QqrKVU9Mc.jpg

 IF analysis of Ki67(AO-03-GB121141)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

13RbpmmG6TucMZB.jpg

 IF analysis of Ki67(AO-03-GB121141)red.
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

14rIUihWYW5uqJF.jpg

 IF analysis of Ki67(AO-03-GB121141)red.
Sample: Human liver cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

15v1DOnoc1ACPg7.jpg

 IF analysis of Ki67(AO-03-GB121141)red.
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

16y16TsBwjhpZXg.jpg

 ICC analysis of Ki67(AO-03-GB121141)red.
Sample: Hela, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 100, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

17TcAvjyK2rYBTW.jpg

 ICC analysis of Ki67(AO-03-GB121141)red.
Sample: NIH-3T3, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 100, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB121141-100
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Hurry! only 10 items left in stock.

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