Anti-PCNA Rabbit pAb
   

Anti-PCNA Rabbit pAb

Antibodies Oncology
AO-03-GB11010-1-100
ATLD2,11010-1,AO-03-GB110101,PCNA,CNA,AO-03-GB11010 1,AO-03-GB110101100,AO-03-GB11010-1,proliferating cell nuclear antige,Proliferating cell nuclear antigen
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Product Information


Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antige

Uniprot ID

P17918

Immunogen

Recombinant protein corresponding to Mouse PCNA

Isotype

IgG

Predicted MW./Observed MW.

29 kDa / 36 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 2000 testis, colon
IHC/IF Human, Mouse 1: 400-1: 2000 lung cancer, ovarian cancer, testis



Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.



Images

01DkF7yXzoLKyNw.jpg

 WB analysis of PCNA(AO-03-GB11010-1).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 5000, RT, 1h.

02Q6dnfIK9mWB9E.jpg

 WB analysis of PCNA(AO-03-GB11010-1).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 5000, RT, 1h.

0356kNbV0MhcbDA.jpg

 IF analysis of PCNA(AO-03-GB11010-1)red.
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.

04rNqn1t02JqJbm.jpg

 IF analysis of PCNA(AO-03-GB11010-1)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11010-1-100
: 10 Items
Hurry! only 10 items left in stock.

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