Recombinant Anti-Osteocalcin antibody (Rabbit mAb) - 50 µL

Product Information

Protein full name

Osteocalcin

Synonyms

BGLAP, BGP, OC, OCN, Osteocalcin, bone gamma-carboxyglutamate protein

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Osteocalcin

Uniprot ID

P86546

Isotype

IgG

Clone Number

SB515

Purity

Affinity purification

Subcellular location

Secreted

Applications

Species

Dilution

Positive Tissue

IHC/IF

Mouse, Rat

1: 500-1: 1500

bone

Storage

Storage

Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

background

Osteocalcin is secreted solely by osteoblasts and thought to play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building, by nature.It is also implicated in bone mineralization and calcium ion homeostasis. Osteocalcin acts as a hormone in the body, causing beta cells in the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin.

Images

IHC analysis of Osteocalcin(GB155684).
Sample: Mouse bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 90°C 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IHC analysis of Osteocalcin(GB155684).
Sample: Rat bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 90°C 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of Osteocalcin(GB155684)red.
Sample: Rat bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 90°C, 30min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.