Recombinant Anti-BMP2 antibody (Rabbit mAb) - 50 µL
   

Recombinant Anti-BMP2 antibody (Rabbit mAb) - 50 µL

Antibodies-Oncology
AO-03-GB15252-50
  • Specification: 50 µL
  • Applications: WB | IHC/IF
  • Species: H,M,R | H,M,R
  • Dilution: 1: 1000-1: 3000 | 1: 500-1: 1000
  • Positive tissue: C2C12, liver, RAW264.7 | colon cancer, bone, endometrial Cancer
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€299.00
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Product Information

Protein full name

Bone morphogenetic protein 2

Synonyms

BMP2, BDA2, BMP2A,bone morphogenetic protein 2

Uniprot ID

P12643

Immunogen

Recombinant protein corresponding to Human BMP2

Isotype

IgG, κ

Clone Number

SB284

Predicted MW./Observed MW.

45 kDa / 前体蛋白：45-50 kDa 二聚体：26-29 kDa; 糖基化单体：18 kDa 单体：12-14 kDa

Purity

Affinity purification

Subcellular location

Secreted

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 1000-1: 3000C2C12, liver, RAW264.7
IHC/IFHuman, Mouse, Rat1: 500-1: 1000colon cancer, bone, endometrial Cancer

Background

Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.

Images

01WEGSTZNUjlTzY.jpg

 WB analysis of BMP2(GB15252).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02DdaypUwRaPUYU.jpg

 IHC analysis of BMP2(GB15252).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

03rVmLgE7J3iIR8.jpg

 IHC analysis of BMP2(GB15252).
Sample: Human endometrial Cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

045cDEaPyZFJ5QB.jpg

 IHC analysis of BMP2(GB15252).
Sample: Mouse bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

05OjYAISUBDa17U.jpg

 IHC analysis of BMP2(GB15252).
Sample: Mouse bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

06An8oJ0e1O5wDT.jpg

 IHC analysis of BMP2(GB15252).
Sample: Rat bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

07IrhX6LTewIuZx.jpg

 IHC analysis of BMP2(GB15252).
Sample: Rat bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

08tlpiUgLkNx1Iy.jpg

 IF analysis of BMP2(GB15252)red.
Sample: Mouse bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

090YVTAQaDcXsCj.jpg

 IF analysis of BMP2(GB15252)red.
Sample: Mouse bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

106yR6mPUrpHqVg.jpg

 IF analysis of BMP2(GB15252)red.
Sample: Rat bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

11JCbGbJNL4x1Kd.jpg

 IF analysis of BMP2(GB15252)red.
Sample: Rat bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB15252-50
: 10 Items
Hurry! only 10 items left in stock.

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