Product Information
Protein full name | Tumor necrosis factor receptor superfamily member 11B |
Synonyms | Osteoclastogenesis inhibitory factor, Tnfrsf11b, Ocif, Opg, PDB5, TR1, TNFRSF11B, OCIF, OPG, PDB5 |
Uniprot ID | O00300 |
Immunogen | Recombinant protein corresponding to Human Osteoprotegerin |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Mouse, Rat | 1: 500-1: 1000 | bone |
Background
Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Images
| IHC analysis of Osteoprotegerin(GB115706). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
| IHC analysis of Osteoprotegerin(GB115706). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
| IHC analysis of Osteoprotegerin(GB115706). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
| IHC analysis of Osteoprotegerin(GB115706). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Datasheet: Download datasheet 1
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