Product Information
Protein full name | Biglycan |
Synonyms | A6NLG9, BGN, Bone/cartilage proteoglycan I, DSPG1, PG-S1, PGI, SLRR1A, SEMDX, Small leucine rich protein 1A, Biglycan proteoglycan, Dermatan sulphate proteoglycan I |
Uniprot ID | P28653 |
Immunogen | Recombinant protein corresponding to Mouse Biglycan |
Isotype | IgG |
Predicted MW./Observed MW. | 42 kDa / 42 kDa |
Purity | Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Rat | 1: 1000-1: 2000 | skeletal muscle, cartilage |
Background
Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family . It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound . The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein . Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule .
Images
| WB analysis of Biglycan(GB113509). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Datasheet: Download datasheet 1
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