Product Information
Protein full name | Transcription factor Sp7 |
Synonyms | C22, Zinc finger protein osterix, Sp7, Osx, Osterix, Sp7 transcription factor |
Uniprot ID | Q8VI67 |
Immunogen | Recombinant protein corresponding to Mouse Sp7 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Mouse, Rat | 1: 200-1: 1000 | bone injury, embryonic mouse, embryonic rat |
Background
This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.
Images
| IHC analysis of Sp7/Osterix(GB111900). Sample: Mouse bone injury, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Sp7/Osterix(GB111900). Sample: Mouse embryonic mouse, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Sp7/Osterix(GB111900). Sample: Rat embryonic rat, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Sp7/Osterix(GB111900). Sample: Rat embryonic rat, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IF analysis of Sp7/Osterix(GB111900)red. Sample: Rat embryonic rat, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Datasheet: Download datasheet 1
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