Product InformationDescriptionBMP2 rabbit polyclonalProtein full nameBone morphogenetic protein 2SynonymsBMP2, BDA2, BMP2A, bone morphogenetic protein 2ImmunogenKLH conjugated Synthetic peptide cor
Product Information
Protein full name | Bone morphogenetic protein 2 |
Synonyms | BMP2, BDA2, BMP2A, bone morphogenetic protein 2 |
Uniprot ID | P21274 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse BMP2 |
Isotype | IgG |
Predicted MW./Observed MW. | 45 kDa / 45 kDa |
Purity | Affinity purification |
Subcellular location | Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, spleen, lung |
| IHC/IF | Human (predicted), Mouse, Rat | 1: 200-1: 1000 | bone |
Background
Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.
Images
| WB analysis of BMP2(GB11252).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
| WB analysis of BMP2(GB11252).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
| IHC analysis of BMP2(GB11252).
Sample: Mouse bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of BMP2(GB11252).
Sample: Mouse bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Datasheet: Download datasheet 1
Français
English
