Anti-Bone Sialoprotein Rabbit pAb - 50 µL
- Specification: 50 µL
- Applications: IHC | IF
- Species: M,R | M,R
- Dilution: 1:500-1:1500 | 1:500-1:1000
- Positive tissue: bone | bone
Product InformationDescriptionBone Sialoprotein rabbit polyclonalProtein full nameBone sialoprotein 2SynonymsIBSP, BNSP, BSP, BSP-II, SP-II, integrin binding sialoproteinImmunogenKLH conjugated Syn
Product Information
Protein full name | Bone sialoprotein 2 |
Synonyms | IBSP, BNSP, BSP, BSP-II, SP-II, Integrin binding sialoprotein |
Uniprot ID | Q61711 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Bone Sialoprotein |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Mouse, Rat | 1: 500-1: 1500 | bone |
Background
Bone sialoprotein (BSP) is a component of mineralized tissues such as bone, dentin, cementum and calcified cartilage. BSP is a significant component of the bone extracellular matrix and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone and cementum. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts.
Images
| IHC analysis of Bone Sialoprotein(GB11588). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Bone Sialoprotein(GB11588). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Bone Sialoprotein(GB11588). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
Datasheet: Download datasheet 1
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