Anti-Bone Sialoprotein Rabbit pAb - 50 µL

AO-03-GB11588-50
  • Specification: 50 µL
  • Applications: IHC | IF
  • Species: M,R | M,R
  • Dilution: 1:500-1:1500 | 1:500-1:1000
  • Positive tissue: bone | bone
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Product InformationDescriptionBone Sialoprotein rabbit polyclonalProtein full nameBone sialoprotein 2SynonymsIBSP, BNSP, BSP, BSP-II, SP-II, integrin binding sialoproteinImmunogenKLH conjugated Syn

Product Information

Protein full name

Bone sialoprotein 2

Synonyms

IBSP, BNSP, BSP, BSP-II, SP-II, Integrin binding sialoprotein

Uniprot ID

Q61711

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Bone Sialoprotein

Isotype

IgG

Purity

Affinity purification

Subcellular location

Secreted

Applications

ApplicationsSpeciesDilutionPositive Tissue
IHC/IFMouse, Rat1: 500-1: 1500bone

Background

Bone sialoprotein (BSP) is a component of mineralized tissues such as bone, dentin, cementum and calcified cartilage. BSP is a significant component of the bone extracellular matrix and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone and cementum. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts.

Images

01Qavr1Th8Qyyfv.jpg

IHC analysis of Bone Sialoprotein(GB11588).
Sample: Mouse bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02nEWTs6tiie80g.jpg

IHC analysis of Bone Sialoprotein(GB11588).
Sample: Rat bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03wbiZQIuXpdo4O.jpg

IHC analysis of Bone Sialoprotein(GB11588).
Sample: Mouse bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

Datasheet: Download datasheet 1

: AO-03-GB11588-50
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