Product Information
Protein full name | Cathepsin L2 |
Synonyms | cathepsin L2, Cathepsin U, Cathepsin V, CATL2, CTSL2, CTSU, CTSV |
Uniprot ID | O60911 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Human Cathepsin V |
Isotype | IgG |
Predicted MW./Observed MW. | 37 kDa / 37 kDa |
Purity | Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Rat | 1: 300-1: 1000 | kidney, testis |
Background
Cathepsin V is a lysosomal cysteine protease which has an important role in corneal physiology.Cathepsin V was found to be differentially expressed in relation to pigmentary skin backgrounds, relative to darkly pigmented skins. It is expressed in primary keratinocytes, melanocytes, and HaCaT keratinocytes, but not in primary fibroblasts. The purified cathepsin L2 was a high molecular weight proteinase of about 78 kDa, but it was unstable to SDS and reducing conditions, which dissociated parts of the proteinase into 66 kDa, 31 kDa and 26 kDa fragments and it can form a complex of 70 kDa with an unidentified subunit.
Images
| WB analysis of Cathepsin V(GB113344). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Datasheet: Download datasheet 1
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