Anti-Cathepsin Z Rabbit pAb - 100 µL
   

Anti-Cathepsin Z Rabbit pAb - 100 µL

Antibodies-Oncology
AO-03-GB112171-100
  • Specification: 100 µL
  • Applications: WB | IHC/IF
  • Species: M,R | M,R
  • Dilution: 1: 500-1: 1000 | 1: 600-1: 1200
  • Positive tissue: lung, spleen, lymph node | liver, rectum
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€429.00
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Product Information

Protein full name

Cathepsin Z

Synonyms

Ctsz, Cathepsin B2, Cathepsin IV, Cathepsin P, Preprocathepsin P, Carboxypeptidase LB, CTSX

Uniprot ID

Q9WUU7

Immunogen

Recombinant protein corresponding to Mouse Cathepsin Z

Isotype

IgG

Predicted MW./Observed MW.

34 kDa / 37 kDa

Purity

Affinity purification

Subcellular location

Lysosome

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBMouse, Rat1: 500-1: 1000lung, spleen, lymph node
IHC/IFMouse, Rat1: 600-1: 1200duodenum, liver, rectum

Background

The protein encoded by this gene is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis.

Images

01TTPDaFYzvpdKt.jpg

 WB analysis of Cathepsin Z(GB112171).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02JoxdTAkcYf0gr.jpg

 WB analysis of Cathepsin Z(GB112171).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

038fMYcrt6WLOzG.jpg

 IHC analysis of Cathepsin Z(GB112171).
Sample: Mouse liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 600, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04y7C9Kc7InjjYF.jpg

 IHC analysis of Cathepsin Z(GB112171).
Sample: Mouse duodenum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 600, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05aUQBzU109LRuo.jpg

 IF analysis of Cathepsin Z(GB112171)red.
Sample: Mouse stomach, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

06zHn79iWgEDjjO.jpg

 IF analysis of Cathepsin Z(GB112171)red.
Sample: Mouse rectum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB112171-100
: 10 Items
Hurry! only 10 items left in stock.

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