Product Information
Protein full name | Cathepsin D |
Synonyms | Ctsd, CatD, CD, CLN10, CPSD, Epididymis secretory sperm binding protein Li 130P, HEL S 130P, Lysosomal aspartyl peptidase |
Uniprot ID | P18242 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Cathepsin D |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Lysosome, Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Human, Mouse | 1: 200-1: 400 | liver |
Background
CTSD(Cathepsin D) is also named as CPSD,belongs to the peptidase A1 family.It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. The prepro-CTSD isoform has a molecular mass of 52 kDa, the pro-CTSD 48 kDa and the mature CTSD 32 kDa. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.Defects in CTSD are the cause of neuronal ceroid lipofuscinosis type 10 (CLN10).This antibody is specific to CTSD.
Images
| IF analysis of Cathepsin D(GB111861)red. Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| IF analysis of Cathepsin D(GB111861)red. Sample: Mouse liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| IF analysis of Cathepsin D(GB111861)red. Sample: Mouse liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Datasheet: Download datasheet 1
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