EasyStep Human APOB(Apolipoprotein B) ELISA Kit
AO-06-ELK093ES-96T
| Cat.No | ELK093ES |
| Product Name | EasyStep Human APOB(Apolipoprotein B) ELISA Kit |
| Reactivity | Human |
| Alternative Names | Apo-B; FLDB; Apo B-100; Apo B-48 |
| Assay Type | Sandwich |
| Sensitivity | 43.2 ng/mL |
| Standard | 12 μg/mL |
| Detection Range | 0.19-12 μg/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOB, and the Human APOB standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human APOB. After TMB substrate solution is added, only those wells that contain Human APOB and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOB in the samples is then determined by comparing the OD of the samples to the standard curve. |

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