EasyStep Human cTnI(Cardiac Troponin I) ELISA Kit
AO-06-ELK057ES-48T
| Cat.No | ELK057ES |
| Product Name | EasyStep Human cTnI(Cardiac Troponin I) ELISA Kit |
| Reactivity | Human |
| Alternative Names | TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Cardiac troponin I; Troponin I |
| Assay Type | Sandwich |
| Sensitivity | 19.6 pg/mL |
| Standard | 25 ng/mL |
| Detection Range | 0.39-25 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Cardiovascular biology; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human cTnI, and the Human cTnI standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human cTnI. After TMB substrate solution is added, only those wells that contain Human cTnI and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human cTnI in the samples is then determined by comparing the OD of the samples to the standard curve. |

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