EasyStep Human EGFR(Epidermal Growth Factor Receptor) ELISA Kit
AO-06-ELK072ES-48T
| Cat.No | ELK072ES |
| Product Name | EasyStep Human EGFR(Epidermal Growth Factor Receptor) ELISA Kit |
| Reactivity | Human |
| Alternative Names | ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
| Assay Type | Sandwich |
| Sensitivity | 25 pg/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | CD & Adhesion molecule;Tumor immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR, and the Human EGFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR. After TMB substrate solution is added, only those wells that contain Human EGFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR in the samples is then determined by comparing the OD of the samples to the standard curve. |

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