EasyStep Human MCP1(Monocyte Chemotactic Protein 1) ELISA Kit
AO-06-ELK071ES-48T
| Cat.No | ELK071ES |
| Product Name | EasyStep Human MCP1(Monocyte Chemotactic Protein 1) ELISA Kit |
| Reactivity | Human |
| Alternative Names | CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE |
| Assay Type | Sandwich |
| Sensitivity | 0.57 pg/mL |
| Standard | 500 pg/mL |
| Detection Range | 7.81-500 pg/mL |
| Sample Type | Serum, plasma, cell culture supernatants |
| Assay Length | 1.5h |
| Research Area | Cytokine;Tumor immunity;Infection immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCP1, and the Human MCP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MCP1. After TMB substrate solution is added, only those wells that contain Human MCP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |

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