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EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
AO-06-ELK033ES-96T
Product Overview
| Cat.No | ELK033ES |
| Product Name | EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit |
| Reactivity | Human |
| Alternative Names | VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor |
| Assay Type | Sandwich |
| Sensitivity | 31 pg/mL |
| Standard | 5000 pg/mL |
| Detection Range | 78.2-5000 pg/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Cytokine;Apoptosis;Tumor immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK033ES-96T
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