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EasyStep Rat NPT(Neopterin) ELISA Kit
AO-06-ELK101ES-48T
Product Overview
| Cat.No | ELK101ES |
| Product Name | EasyStep Rat NPT(Neopterin) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | Neopterin |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.4 nmol/L |
| Standard | 30 nmol/L |
| Detection Range | 0.469-30 nmol/L |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Cytokine;Infection immunity; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPT antigen, and the Rat NPT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat NPT to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat NPT in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
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