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EasyStep Rat VEGFR2(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
AO-06-ELK083ES-48T
Product Overview
| Cat.No | ELK083ES |
| Product Name | EasyStep Rat VEGFR2(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | CD309; FLK1; VEGFR; KDR; A Type III Receptor Tyrosine Kinase; Kinase Insert Domain Receptor; Kinase Insert Domain Receptor; Fetal Liver Kinase-1 |
| Assay Type | Sandwich |
| Sensitivity | 157.5 pg/mL |
| Standard | 50 ng/mL |
| Detection Range | 0.78-50 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Signal transduction;CD & Adhesion molecule;Tumor immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFR2, and the Rat VEGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat VEGFR2. After TMB substrate solution is added, only those wells that contain Rat VEGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VEGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
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