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EasyStep Rat vWF(Von Willebrand Factor) ELISA Kit
AO-06-ELK076ES-96T
Product Overview
| Cat.No | ELK076ES |
| Product Name | EasyStep Rat vWF(Von Willebrand Factor) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | F8VWF; VWD; von Willebrand antigen 2 |
| Assay Type | Sandwich |
| Sensitivity | 41 ng/mL |
| Standard | 10 µg/mL |
| Detection Range | 0.16-10 µg/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Signal transduction;Hematology; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat vWF, and the Rat vWF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat vWF. After TMB substrate solution is added, only those wells that contain Rat vWF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat vWF in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
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