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Human IFNa/bR2(Interferon Alpha/Beta Receptor 2) ELISA Kit
AO-06-ELK3549-96T
Product Overview
| Cat.No | ELK3549 |
| Product Name | Human IFNa/bR2(Interferon Alpha/Beta Receptor 2) ELISA Kit |
| Reactivity | Human |
| Alternative Names | IFNαR2; IFN-alpha-REC; IFN-R; IFNαBR; IFNαRB; Interferon(Alpha,Beta And Omega)Receptor Beta Chain; Interferon alpha binding protein; Type I interferon receptor 2 |
| Assay Type | Sandwich |
| Sensitivity | 0.25 ng/mL |
| Standard | 40 ng/mL |
| Detection Range | 0.63-40 ng/mL |
| Sample Type | Serum, plasma, urine and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Infection immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNa/bR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNa/bR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNa/bR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNa/bR2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK3549-96T
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