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Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit
AO-06-ELK2178MS-48T
Product Overview
| Cat.No | ELK2178MS |
| Product Name | Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit |
| Reactivity | Human |
| Alternative Names | IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha |
| Assay Type | Sandwich |
| Sensitivity | 6.5 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK2178MS-48T
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