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Human IL-37(Interleukin 37) Microsample ELISA Kit
AO-06-ELK1343MS-48T
Product Overview
| Cat.No | ELK1343MS |
| Product Name | Human IL-37(Interleukin 37) Microsample ELISA Kit |
| Reactivity | Human |
| Alternative Names | IL-1Z;IL1 Zeta; IL1F7; IL37; FIL1; FIL1(ZETA); FIL1Z; IL-1F7; IL-1H4; IL-1RP1; IL1H4; IL1RP1; Interleukin 37; Interleukin-1-Related Protein; Interleukin 1 Family; Member 7 |
| Assay Type | Sandwich |
| Sensitivity | 3.3 pg/mL |
| Standard | 500 pg/mL |
| Detection Range | 7.82-500 pg/mL |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Cytokine; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-37 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK1343MS-48T
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