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Human IL-6R(Interleukin 6 Receptor) ELISA Kit
AO-06-ELK11855-96T
Product Overview
| Cat.No | ELK11855 |
| Product Name | Human IL-6R(Interleukin 6 Receptor) ELISA Kit |
| Reactivity | Human |
| Alternative Names | IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; |
| Assay Type | Sandwich |
| Sensitivity | 0.45 ng/mL |
| Standard | 30 ng/mL |
| Detection Range | 0.47-30 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 3.5h |
| Research Area | Signal transduction;CD & Adhesion molecule;Infection immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-6R in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK11855-96T
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