Human NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit

Human NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit

ELK Biotechnology
AO-06-ELK2228MS-48T

Human NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit 48T

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Product Overview

Cat.No ELK2228MS
Product Name Human NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit
Reactivity Human
Alternative Names NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B
Assay Type Sandwich
Sensitivity 0.058 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length 3.5h
Research Area Signal transduction;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK2228MS-b-1.jpg
: AO-06-ELK2228MS-48T
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Hurry! only 10 items left in stock.

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