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Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
AO-06-ELK1212-96T
Product Overview
| Cat.No | ELK1212 |
| Product Name | Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit |
| Reactivity | Human |
| Alternative Names | NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb |
| Assay Type | Sandwich |
| Sensitivity | 15 pg/mL |
| Standard | 2500 pg/mL |
| Detection Range | 39.07-2500 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Endocrinology;Cardiovascular biology; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK1212-96T
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