Human RGC32(Response Gene To Complement 32) ELISA Kit

Human RGC32(Response Gene To Complement 32) ELISA Kit

AO-06-ELK6515-48T

Human RGC32(Response Gene To Complement 32) ELISA Kit 48T

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Product Overview

Cat.No ELK6515
Product Name Human RGC32(Response Gene To Complement 32) ELISA Kit
Reactivity Human
Alternative Names RGCC; C13orf15; Regulator of cell cycle RGCC
Assay Type Sandwich
Sensitivity 0.126 ng/mL
Standard 20 ng/mL
Detection Range 0.32-20 ng/mL
Sample Type Tissue homogenates, cell lysates and other biological fluids.
Assay Length 3.5h
Research Area Signal transduction;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RGC32. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RGC32. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RGC32, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RGC32 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK6515-b-1.jpg
: AO-06-ELK6515-48T
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Hurry! only 10 items left in stock.

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