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Human SAM68(Src associated in mitosis 68 kDa protein) ELISA Kit
AO-06-ELK9665-48T
Product Overview
| Cat.No | ELK9665 |
| Product Name | Human SAM68(Src associated in mitosis 68 kDa protein) ELISA Kit |
| Reactivity | Human |
| Alternative Names | KHDRBS1; SAM68; KH domain-containing; RNA-binding, signal transduction-associated protein 1; GAP-associated tyrosine phosphoprotein p62; Src-associated in mitosis 68 kDa protein; Sam68; p21 Ras GTPase-activating protein-associated p62; p68 |
| Assay Type | Sandwich |
| Sensitivity | 0.051 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction; Epigenetics and Nuclear Signaling |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SAM68. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SAM68. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SAM68, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAM68 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK9665-48T
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