Français
English
Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit
AO-06-ELK0778-96T
Product Overview
| Cat.No | ELK0778 |
| Product Name | Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit |
| Reactivity | Human |
| Alternative Names | 2,3DPG |
| Assay Type | Sandwich |
| Sensitivity | 0.14 µmol/mL |
| Standard | 20 µmol/mL |
| Detection Range | 0.32-20 µmol/mL |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Metabolic pathway |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK0778-96T
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package