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Human 3-MH(3-Methylhistidine) ELISA Kit
AO-06-ELK11259-48T
Product Overview
| Cat.No | ELK11259 |
| Product Name | Human 3-MH(3-Methylhistidine) ELISA Kit |
| Reactivity | Human |
| Alternative Names | 3MH |
| Assay Type | Competitive Inhibition |
| Sensitivity | 2.61 nmol/mL |
| Standard | 400 nmol/mL |
| Detection Range | 6.25-400 nmol/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 2h |
| Research Area | Metabolism |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 3-MH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 3-MH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 3-MH in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK11259-48T
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