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Human 3-NT(3-Nitrotyrosine) ELISA Kit
AO-06-ELK11827-48T
Product Overview
| Cat.No | ELK11827 |
| Product Name | Human 3-NT(3-Nitrotyrosine) ELISA Kit |
| Reactivity | Human |
| Alternative Names | 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine |
| Assay Type | Competitive Inhibition |
| Sensitivity | 1.35 ng/mL |
| Standard | 300 ng/mL |
| Detection Range | 4.69-300 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Metabolic pathway;Infection immunity;Rheumatology; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 3-NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK11827-48T
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