Human aCTx(Alpha-Crosslaps) ELISA Kit
AO-06-ELK4853-48T
| Cat.No | ELK4853 |
| Product Name | Human aCTx(Alpha-Crosslaps) ELISA Kit |
| Reactivity | Human |
| Alternative Names | Alpha CTx; a-CTXI; aCTX-I; A-Cr; Type I Collagen C-Telopeptide-Related Fraction |
| Assay Type | Competitive Inhibition |
| Sensitivity | 25.7 pg/mL |
| Standard | 5000 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample Type | serum, plasma, other biological fluids |
| Assay Length | 2h |
| Research Area | Bone metabolism; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human aCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aCTx in the samples is then determined by comparing the OD of the samples to the standard curve. |

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