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Human Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
AO-06-ELK2631-96T
Product Overview
| Cat.No | ELK2631 |
| Product Name | Human Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit |
| Reactivity | Human |
| Alternative Names | ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase |
| Assay Type | Sandwich |
| Sensitivity | 0.109 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase;Neuro science; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK2631-96T
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