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Human B2-glycoprotein 1 (β2-GP1) antibody lgA ELISA Kit
AO-06-ELK0937-96T
Product Overview
| Cat.No | ELK0937 |
| Product Name | Human B2-glycoprotein 1 (β2-GP1) antibody lgA ELISA Kit |
| Reactivity | Human |
| Alternative Names | APOH; BG; B2G1; B2GP1 |
| Assay Type | Sandwich |
| Sensitivity | 26.8 pg/mL |
| Standard | 4000 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Immune molecule; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human β2-GP1 lgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human β2-GP1 lgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human β2-GP1 lgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human β2-GP1 lgA in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK0937-96T
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