Français
English
Human BAD(Bcl2 antagonist of cell death) ELISA Kit
AO-06-ELK9531-48T
Product Overview
| Cat.No | ELK9531 |
| Product Name | Human BAD(Bcl2 antagonist of cell death) ELISA Kit |
| Reactivity | Human |
| Alternative Names | LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohyd犬e-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29 |
| Assay Type | Sandwich |
| Sensitivity | 0.056 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Cell Biology; Cancer; Metabolism |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BAD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BAD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BAD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BAD in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package