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Product Overview
| Cat.No | ELK8382 |
| Product Name | Human Barbiturates ELISA Kit |
| Reactivity | Human |
| Alternative Names | Barbituates |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.1 ng/mL |
| Standard | 25 ng/mL |
| Detection Range | 0.4-25 ng/mL |
| Sample Type | Plasma, Serum, Urine |
| Assay Length | 2h |
| Research Area | Neuro science; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Barbitues. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Barbitues. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Barbitues in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK8382-96T
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