Human bCTx(Beta-Crosslaps) ELISA Kit
AO-06-ELK1544-48T
| Cat.No | ELK1544 |
| Product Name | Human bCTx(Beta-Crosslaps) ELISA Kit |
| Reactivity | Human |
| Alternative Names | Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction |
| Assay Type | Competitive Inhibition |
| Sensitivity | 36.16 pg/mL |
| Standard | 8000 pg/mL |
| Detection Range | 125-8000 pg/mL |
| Sample Type | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2h |
| Research Area | Bone metabolism; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human bCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human bCTx in the samples is then determined by comparing the OD of the samples to the standard curve. |

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